LC-MS/MS Service

Quantification of small molecules by liquid chromatography coupled to a tandem mass spectrometer (LC-MS/MS) provides critical quantitative analytical support in both the drug development process and medical research.

Total Scientific Limited (TSL) have extensive expertise in the development, validation and use of LC-MS/MS assays, using a triple-quadrupole instrument to perform quantitative analysis in a variety of sample matrices. Matrices previously quantitated by staff at TSL include, but are not limited to, urine, plasma, tissue (brain, tumour, liver spleen), cells, cell media, cerebral spinal fluid, solutions and formulations. A variety of analytes, both small molecules and biologicals, can be quantified by LC-MS/MS.

These analyses can be used for pharmacokinetic, pharmacodynamic or toxicology applications. We are currently working towards accreditation for GLP and GCLP, and will then be able to support not only research applications but regulated preclinical and clinical studies.

The LC-MS/MS platform adopted by TSL is a Waters® Xevo® TQ-XS triple quadrupole mass spectrometer coupled to a Waters Acquity UPLC® I-Class system. The combination of a fast scanning mass spectrometer with an Ultra High Performance Chromatography Liquid Chromatography separation system (UHPLC) allows rapid high resolution separations without compromising detection.

Please contact us for a no obligation discussion if you would like to know more about Total Scientific’s LC-MS/MS service and how we may be able to assist your company/institution’s research studies.